Getting DNA from wheat
- First, I added 20 ml warm water to the wheat and mix for 3 minutes . I noticed the wheat got very thick. then after i added 1 ml (1/4 teaspoon) of detergent and mix gently every five minutes. It looked like watery bread crumbs and it also turned more yellow. The i used a pipette to remove foam from the top of the solution After that i put 14 ml of alcohol down the side so that it forms a layer on the top of the water. It helped the DNA get out from the wheat. Next, i let it sit for a few minutes and white stringily DNA appeared where the water meet the alcohol. Then i used a little wooden stick to to remove the DNA. We thought the over all DNA looked like a booger! Ahaha....it formed into to four layers 1st- the wheat 2nd- the detergent 3rd- white layer (dna) 4th- alcohol *
No comments:
Post a Comment